human duo set elisa kits  (R&D Systems)

Journal: Redox Biology

Article Title: C-terminal interleukin 1 alpha (IL-1α) overexpression drives EMT and a vulnerability to ferroptosis in HNSCC

doi: 10.1016/j.redox.2026.104172

Figure Lengend Snippet: Characterization of IL-1α overexpression constructs in Cal27 HNSCC cells. Gene expression (A-C), protein release (D) and protein expression (E-G) of the three IL1A constructs – Full- Length (FL), N-terminal (NT) and C-terminal (CT) in the Cal27 IL-1α-overexpressing cells were analyzed by RT-qPCR, ELISA and Western blot respectively compared to control. GAPDH was used as an endogenous control for PCR analysis (A-C); α-Tubulin was used as the loading control for whole cell lysate and cytoplasmic fraction western blots (E-F); and Lamin B1 was used as the loading control for the nuclear fraction Western blot (G). Bars represent mean ± SEM from n = 3 independent experiments. IL-1α band intensities were normalized to the respective loading controls. ∗p < 0.05; ∗∗p < 0.01; ∗∗∗p < 0.001; ∗∗∗∗p < 0.0001.

Article Snippet: Cell culture media from IL-1α overexpressed Cal27 cells were collected for analyzing the levels of IL-1α, IL-1β, IL-6, IL-8 or IL-1RA using Human Duo Set ELISA kits (R&D Systems, Minneapolis, MN) according to the manufacturer's protocols.

Techniques: Over Expression, Construct, Gene Expression, Expressing, Quantitative RT-PCR, Enzyme-linked Immunosorbent Assay, Western Blot, Control

Journal: Redox Biology

Article Title: C-terminal interleukin 1 alpha (IL-1α) overexpression drives EMT and a vulnerability to ferroptosis in HNSCC

doi: 10.1016/j.redox.2026.104172

Figure Lengend Snippet: IL-1α overexpression alters expression of IL-1 pathway genes. Gene expression of IL1B (A), IL6 (B), IL8 (C), IL1RN (D), IL1RAP (E) and IL1R1 (F) in the three IL1A constructs – Full-Length (FL), N-terminal (NT) and C-terminal (CT) in Cal27 IL-1αoverexpressing cells were analyzed by RT-PCR using GAPDH as an endogenous control. Protein secretion of IL-1β (G), IL-6 (H), IL-8 (I), and IL1RA (J) in cell culture supernatants was quantified by ELISA with protein concentrations normalized to cell numbers. Whole cell lysates were analyzed for IL-1R1 expression by Western blot using GAPDH as a loading control (K). Bars represent mean ± SEM from n = 3 independent experiments.∗p < 0.05; ∗∗p < 0.01; ∗∗∗p < 0.001; ∗∗∗∗p < 0.0001.

Article Snippet: Cell culture media from IL-1α overexpressed Cal27 cells were collected for analyzing the levels of IL-1α, IL-1β, IL-6, IL-8 or IL-1RA using Human Duo Set ELISA kits (R&D Systems, Minneapolis, MN) according to the manufacturer's protocols.

Techniques: Over Expression, Expressing, Gene Expression, Construct, Reverse Transcription Polymerase Chain Reaction, Control, Cell Culture, Enzyme-linked Immunosorbent Assay, Western Blot

Journal: bioRxiv

Article Title: Chronic NLRP3 inflammasome activation drives neutrophil brain entry and interactions with microglia

doi: 10.64898/2026.04.22.720282

Figure Lengend Snippet: hNLRP3 D305N mice show constitutive inflammasome activity in the brain, broad neuroinflammatory signaling, and signs of BBB permeability and neuronal damage (a) IL-1β MSD and IL-1RA ELISA quantification of bulk brain samples (n = 6-13/group). (b-c) Bulk brain samples were assayed in a cytokine panel. Select cytokines are shown in column graphs (n = 3-13/group) and the full panel is shown as heatmap (columns represent individual mice). Heatmap values are normalized to hNLRP3 WT . (d-e) Representative images and quantification of western blot for IgG heavy chain (HC) and IgG light chain (LC) in bulk brain samples. Vinculin is used as loading control. Normal two-way ANOVA with Tukey multiple testing correction for comparisons across genotype (n = 5-7/group). (f) Nf-L Simoa assay quantification in CSF samples (n = 6-13/group). All panels include hNLRP3 WT , mNlrp3 WT , and hNLRP3 D305N mice. Unless otherwise noted, lognormal two-way ANOVA with Tukey multiple testing correction for comparisons across genotype and age group. *p adj < 0.05, **p adj < 0.01, ***p adj < 0.001. Data are shown as mean ± SD. Full source data and statistic values in Source Data.

Article Snippet: R&D Systems Mouse IL-1ra/IL-1F3 Quantikine ELISA Kit was used to assess IL-1RA levels and MBL Mouse IL-18 ELISA Kit was used to assess IL-18 levels.

Techniques: Activity Assay, Permeability, Enzyme-linked Immunosorbent Assay, Western Blot, Control